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These ACUPs are produced and enforced to ensure the welfare of animals used in research and teaching at Cornell University. They ensure that high quality care is provided for all research animals, and that they are used in compliance with federal, state, and local regulations and guidelines. Cornell faculty, researchers and their staff, animal facility personnel, and students must comply with these ACUPs. Any exemption must be approved by the Institutional Animal Care and Use Committee (IACUC) prior to its application.

Please feel free to reference and use these ACUPs as needed but if you reference them in lectures, publications, etc. give credit where credit is due. Thank you.

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These ACUPs are in PDF format; you can download a free reader from Adobe.

Anesthesia and Analgesia

101 Rodent Anesthesia
102 Analgesia
103 Rabbit Anesthesia
104 Cat and Dog Anesthesia
110 Fish and Amphibian Anesthesia


201 Rodent Surgery
207 Preparation and Care of Fistulated Bovine
208 Mouse Tail Snipping
209 Non-Rodent Surgery
210 Non-Survival Surgery


301 Rodent Euthanasia
306 Fish and Amphibian Euthanasia
307 Euthanasia of Non-Rodent Mammals
308 Avian Euthanasia
309 Maintenance of Decapitation Equipment

Experimental Guidelines

401 Recommended Volumes for Administered Substances
402 Humane Intervention Points
403 Recommended Blood Collection Volume and Frequency
408 Retro-Orbital Bleeding
411 Monoclonal Antibody Production
412 Oocyte Harvesting from Amphibians
413 Use of Non-Pharmaceutical Grade Compounds in Research Animals

Animal Care

509 Animal Acquisition, Receiving, and Acclimation
513 Rodent Husbandry and Breeding
514 Rabbit Husbandry
515 Dog Husbandry
516 Cat Husbandry
517 Swine Husbandry
518 Dairy Cattle Husbandry
520 Sheep Husbandry
532 Cleaning and Sanitizing Materials Used in the Transport of Animals
538 Vermin Program
539 Equine Husbandry
542 Maintaining Health and Procedure Records for Research and Teaching Animals
546 Socialization and Exercise for Dogs in Research Facilities
547 Animal Transport Outside Animal Facilities
548 Beef Cattle Husbandry
549 Poultry Husbandry
550 Duck Husbandry and Preventative Health Program
551 Monitoring of Humidity and Temperature in Agricultural Facilities
552 Mouse Identification
554 Performance of Ruminant Tissue Biopsies
560 Environmental Enrichment Program for Rodents and Rabbits

Animal Health and Preventative Care

604 Preventative Health Program for Dogs
605 Preventative Health Program for Cats
607 Reporting Sick Animals
609 Equid Acquisition, Receiving, Acclimation, and Disposition
618 Perinatal Care of the Dog
619 Tumor and Cell Line Testing
621 Quarantine and Entrance Diagnostics of Passeriformes
622 Preventative Health Program for Equids
623 Fish Health Monitoring Program

Occupational Health and Safety

707 Animal Related Injury
710 Working with Wild Rodents
712 Waste Anesthetic Gas Scavenging Systems
715 Personal Protective Equipment
716 Use of Animals in Public Displays
718 Safety Guidelines for Fieldwork


805 CARE Animal Transfers
806 Anesthesia Administered by CUHA or CARE
807 Adoption Program Policy